Team:Washington/Team/Members

From 2011.igem.org

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== '''Who we are''' ==
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__NOTOC__
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<gallery caption="Undergraduate Team Members" widths="180px" heights="120px" perrow="3">
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<!---------------------------------------PAGE CONTENT GOES BELOW THIS---------------------------------------->
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Image:Washington_jeff_johnson.png|<center>Jeff Johnson</center>
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Image:chris_eiben.jpg|<center>Chris Eiben</center>
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Image:Washington_Huber_Tomas.jpg|<center>Tomas Huber</center>
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Image:Washington_Ivan_Huang.jpg|<center>Ivan Huang</center>
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Image:Washington_Peixian.JPG‎|<center>Peixian Liu</center>
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Image:Washington_Denny_Luan.JPG|<center>Denny Luan</center>
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Image:Wu_Cindy.jpg|<center>Cindy Wu</center>
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Image:Washington_Sean_Wu.jpg|<center>Sean Wu</center>
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Image:Washington_david_zong.jpg‎|<center>David Zong</center>
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Image:Laura_Austin.jpg|<center>Laura Austin</center>
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Image:me2.jpg|<center>Matt Coyne</center>
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Image:Washington_Justin.jpg|<center>Justin De Leon</center>
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Image:Matthew_Harger.JPG|<center>Matthew Harger</center>
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Image:DSC06208.JPG|<center>Jesa Landis</center>
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</gallery>
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<center><big><big><big><big>'''Who we are'''</big></big></big></big></center><br>
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<center>
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<gallery caption="Undergraduate Team Members" widths="180px" heights="120px" perrow="4">
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Image:Washington Casey Ager Profile1.jpg‎|<center>Casey Ager <br/> Biochemistry</center>
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Image:Washington Photo-0088A.png|<center>Juhye An <br/> Biochemistry </center>
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Image: Profile_Michael_Brasino.jpg‎|<center>Michael Brasino <br/> Materials Science and Engineering</center>
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Image:Washington MC profilepic1.jpg|<center>Marika Cheng <br/> Microbiology</center>
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Image:Washington_choe_chris.png|<center>Chris Choe <br/> Undecided</center>
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Image:Washington DeLeon 2011.jpg|<center>Justin De Leon <br/> Microbiology</center>
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File:Princess Peachy.JPG|<center>Sydney Gordon <br/> Biochemistry, Music</center>
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Image:CIMG0021.jpg|<center>Daniel Hadidi <br/> Neurobiology</center>
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Image:Washington_Matthew_Harger.JPG|<center>Matthew Harger <br/> Cellular and Molecular Biology</center>
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Image:Washington_Elaine.jpg|<center>Elaine Lai <br/> Microbiology, Chemistry</center>
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Image:Washington_2011_Lab_Ben.png|<center>Benjamin Mo <br/> Bioengineering</center>
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Image:Washington 2011 CIMG0015-A.jpg|<center>Austin Moon <br/> Molecular Biology</center>
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Image:Washington_iGEM2011_Rashmi.jpg|<center>Rashmi Ravichandran <br/> Microbiology</center>
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Image:rsz_cats.png|<center>Seth Sagulo <br/> Biochemistry</center>
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File:Washington_Liz.png|<center>Liz Stanley <br/> Microbiology, Chemistry</center>
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Image:Washington_Angus_Pic.jpg|<center>Angus Toland <br/> Microbiology</center>
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Image:Washington_Sarah.jpg|<center>Sarah Wolf <br/> Biochemistry</center>
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Image:Washington_igem11whowearepicture.jpg|<center>SauShun (Alicia) Wong <br/> Materials Science and Engineering</center>
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Image:Washington_Cindy.jpg|<center>Cindy Wu <br/> Cellular and Molecular Biology</center>
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Image:Washington_Sean_Wu.jpg|<center>Sean Wu <br/> Computer Science and Engineering</center>
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File:Washington_EmilyYang.jpg|<center>Emily Yang <br/> Bioengineering</center>
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Image:Washington_lei_zheg.png|<center>Lei Zheng <br/> Biochemistry</center>
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Image:Washington_david_zong.jpg‎|<center>David Zong <br/> Bioengineering</center>
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<gallery caption="Graduate Advisors" widths="180px" heights="120px" perrow="3">
 
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Image:Washington_matt_smith.png|<center>Matt Smith <br/> Molecular and Cellular Biology</center>
 
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Image:Ingrid.jpg|<center>Ingrid Swanson <br/> Microbiology <br/></center>
 
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Image:Regbert.jpg|<center>Rob Egbert <br/> Electrical Engineering</center>
 
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Image:galdzicki.jpg|<center>Michal Galdzicki <br/> Biomedical and Health Informatics</center>
 
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Image:Washington_Siegel_2010.jpg|<center>Justin Siegel <br/> Biomolecular Structure and Design</center>
 
</gallery>
</gallery>
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<gallery caption="Advisors" widths="180px" heights="120px" perrow="4">
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File:Aaron_bike_UW.png|<center>Aaron Chevalier <br/> Bioengineering </center>
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Image:Regbert.jpg|<center>Rob Egbert <br/> Electrical Engineering </center>
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Image:CEiben.jpg|<center>Chris Eiben <br/> Biochemistry </center>
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Image:IMG_0129.jpg|<center>Jeremy Mills <br/> Biochemistry </center>
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Image:Siegel_UW_2011.jpg|<center>Justin Siegel <br/> Biomolecular Structure and Design</center>
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Image:Washington2011_Matthew-d-smith.jpg‎|<center>Matt Smith <br/> Molecular and Cellular Biology</center>
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File:Washington_Ingrid_Pic_1.jpg|<center>Ingrid Swanson Pultz<br/> Microbiology </center>
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</gallery>
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<gallery caption="Faculty Advisors" widths="180px" heights="120px" perrow="3">
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<gallery caption="Faculty" widths="180px" heights="120px" perrow="5">
image:Washington_David_Baker.jpg|<center>David Baker <br/> Biochemistry</center>
image:Washington_David_Baker.jpg|<center>David Baker <br/> Biochemistry</center>
Image:Washington_Klavins.jpg|<center>Eric Klavins <br/> Electrical Engineering</center>
Image:Washington_Klavins.jpg|<center>Eric Klavins <br/> Electrical Engineering</center>
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image:Washington_Mougous.jpg|<center>Joseph Mougous <br/> Microbiology</center>
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File:Washington_Sauro.png|<center>Herbert Sauro <br/>Bioengineering</center>
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Image:Washington_Sauro.jpg|<center>Herbert Sauro <br/> Bioengineering</center>
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</gallery>
</gallery>
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<gallery caption="Collaborators and Support" widths="180px" heights="120px" perrow="3">
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<gallery caption="Collaborators and Support" widths="140px" heights="100px" perrow="5">
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Image:Washington_Biochemistry.gif|<center>Biochemistry <br/> Lab space</center>
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image:Washington_uwmb_header.jpg|<center>Microbiology <br/> Financial support and lab space</center>
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Image:Wahsington_Fisher_Logo.jpg|<center>Fisher <br/> Lab Materials</center>
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Image:Washington_Arthur_Friedlander.jpg|<center>Arthur Friedlander @ USAMRIID <br/> Help planning the project and offering to test our new enzymes' effectiveness against Anthrax!</center>
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Image:Washington_Chandran.png|<center>Deepak Chandran @ Bioengineering, University of Washington<br/> Always available to answer our TinkerCell API questions no matter how trivial.</center>
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</gallery>
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Image:Washington_OSLI.png|<center><b>Oil Sands Leadership Initiative</b><br/>Funding to support Travel and Registration Costs</center>
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Image:Washington_UniversitySeal.gif|<center><b>UW Biochemistry</b><br/>Lab space</center>
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Image:Washington_Anaspec.gif|<center><b>Anaspec</b><br/>Peptide Discounts</center>
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Image:Washington_ARPA-E_Logo.png|<center><b>Advanced Research Projects Agency - Energy</b><br/>Registration Support</center>
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Image:Washington2011_Hhmi_362_72.jpg|<center><b>Howard Hughes Medical Institute</b><br/>Lab materials and supplies</center>
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</gallery>
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</center>
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<br>
== '''Who did what''' ==
== '''Who did what''' ==
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The teams were assembled during our winter quarter.  During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures.  During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out.  Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.
 
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=== The Gram Positive Therapeutic ===
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The teams were assembled during our winter quarter. During this term, we went around to classes and had weekly meetings to introduce students to synthetic biology through a series of guest lectures. During the spring, students participated in brainstorming projects related to the sponsoring Faculty advisors' labs (Baker and Klavins) and came up with the project they wanted to carry out. Students also planned and participated in community outreach events where we taught the local public about synthetic biology. Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.
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Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants.
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'''The work documented here in this wiki and on our presentation is entirely the work of iGEM students - it does not represent the project of any host lab, advisor, or instructor participating in iGEM.'''
 +
 
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=== Diesel Production ===
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 +
After producing promising results, in future directions:
 +
 
 +
Casey Ager, Austin Moon, and Seth Sagulo worked on Enzyme Localization via Direct Fusion and Zinc Finger Fusion methods.
 +
 
 +
Juhye An, Elaine Lai, and Benjamin Mo worked on Decarbonylase Redesign
 +
 
 +
Marika Cheng and Justin De Leon worked on Alternative Chassis
 +
 
 +
Chris Choe and David Zong worked on Alternate Aldehyde Production
 +
 
 +
Matthew Harger worked on Branched Alkanes Production
 +
 
 +
Matthew Harger and Lei Zheng worked on System Optimization
 +
 
 +
Emily Yang worked on modelling the alkane production pathway.
 +
 
 +
=== Gluten Destruction ===
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Sydney Gordon, Daniel Hadidi, Liz Stanley, Angus Toland, Sarah Wolf, and Sean Wu designed, built, and tested Kumamolisin-As and over 100 mutants to combat gluten intolerance by increasing the activity on the PQLP antigenic peptide.
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=== The Gram Negative Therapeutic ===
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=== iGEM Toolkits ===
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Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts.
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Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.
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Michael Brasino, Rashmi Ravichandran, and Alicia Wong worked on extracting and BioBricking the essential genes for magnetosomes, made the fusion proteins, and did the experimental measurements.  They also expanded on work that was started by UW iGEM students in 2010, by constructing more Gibson-friendly plasmid backbones and characterizing the Gibson-assembly efficiencies of pSB and pGA plasmids.
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=== New Tools ===
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=== Community Outreach ===
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Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_68:_Standard_for_the_Electronic_Distribution_of_SBOLv_Diagrams BBF RFC 68]. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document.
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Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen.  He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks.
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Cindy Wu headed and organized all iGEM 2011 Community Outreach events.

Latest revision as of 06:04, 28 October 2011


Who we are

Undergraduate Team Members
Casey Ager
Biochemistry
Juhye An
Biochemistry
Michael Brasino
Materials Science and Engineering
Marika Cheng
Microbiology
Chris Choe
Undecided
Justin De Leon
Microbiology
Sydney Gordon
Biochemistry, Music
Daniel Hadidi
Neurobiology
Matthew Harger
Cellular and Molecular Biology
Elaine Lai
Microbiology, Chemistry
Benjamin Mo
Bioengineering
Austin Moon
Molecular Biology
Rashmi Ravichandran
Microbiology
Seth Sagulo
Biochemistry
Liz Stanley
Microbiology, Chemistry
Angus Toland
Microbiology
Sarah Wolf
Biochemistry
SauShun (Alicia) Wong
Materials Science and Engineering
Cindy Wu
Cellular and Molecular Biology
Sean Wu
Computer Science and Engineering
Emily Yang
Bioengineering
Lei Zheng
Biochemistry
David Zong
Bioengineering


Advisors
Aaron Chevalier
Bioengineering
Rob Egbert
Electrical Engineering
Chris Eiben
Biochemistry
Jeremy Mills
Biochemistry
Justin Siegel
Biomolecular Structure and Design
Matt Smith
Molecular and Cellular Biology
Ingrid Swanson Pultz
Microbiology


Faculty
David Baker
Biochemistry
Eric Klavins
Electrical Engineering
Herbert Sauro
Bioengineering


Collaborators and Support
Oil Sands Leadership Initiative
Funding to support Travel and Registration Costs
Washington UniversitySeal.gif
UW Biochemistry
Lab space
Washington Anaspec.gif
Anaspec
Peptide Discounts
Advanced Research Projects Agency - Energy
Registration Support
Howard Hughes Medical Institute
Lab materials and supplies


Who did what

The teams were assembled during our winter quarter. During this term, we went around to classes and had weekly meetings to introduce students to synthetic biology through a series of guest lectures. During the spring, students participated in brainstorming projects related to the sponsoring Faculty advisors' labs (Baker and Klavins) and came up with the project they wanted to carry out. Students also planned and participated in community outreach events where we taught the local public about synthetic biology. Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.

The work documented here in this wiki and on our presentation is entirely the work of iGEM students - it does not represent the project of any host lab, advisor, or instructor participating in iGEM.

Diesel Production

After producing promising results, in future directions:

Casey Ager, Austin Moon, and Seth Sagulo worked on Enzyme Localization via Direct Fusion and Zinc Finger Fusion methods.

Juhye An, Elaine Lai, and Benjamin Mo worked on Decarbonylase Redesign

Marika Cheng and Justin De Leon worked on Alternative Chassis

Chris Choe and David Zong worked on Alternate Aldehyde Production

Matthew Harger worked on Branched Alkanes Production

Matthew Harger and Lei Zheng worked on System Optimization

Emily Yang worked on modelling the alkane production pathway.

Gluten Destruction

Sydney Gordon, Daniel Hadidi, Liz Stanley, Angus Toland, Sarah Wolf, and Sean Wu designed, built, and tested Kumamolisin-As and over 100 mutants to combat gluten intolerance by increasing the activity on the PQLP antigenic peptide.

iGEM Toolkits

Michael Brasino, Rashmi Ravichandran, and Alicia Wong worked on extracting and BioBricking the essential genes for magnetosomes, made the fusion proteins, and did the experimental measurements. They also expanded on work that was started by UW iGEM students in 2010, by constructing more Gibson-friendly plasmid backbones and characterizing the Gibson-assembly efficiencies of pSB and pGA plasmids.

Community Outreach

Cindy Wu headed and organized all iGEM 2011 Community Outreach events.

Retrieved from "http://2011.igem.org/Team:Washington/Team/Members"