Team:Washington/Protocols/Purified Enzyme Assay


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(Whole Cell Lysate Assay)
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=Whole Cell Lysate Assay=
=Purified Enzyme Assay=

Revision as of 01:11, 23 September 2011

Purified Enzyme Assay

  • Assay
    • Add 90uL of 5uM substrate (in NaAc pH 4.0 buffer) to each well of a black fluorescent microtiter assay plate
    • Start reaction by adding 0.0125mg/mL enzyme (try to avoid bubbles and pippette quickly, but accurately)
      • Use the P20 multichannel with a taped cardboard stopper to make sure you don't hit the pellet!
    • Monitor the reaction with the SpectraMax

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