Team:Washington/Protocols/PCR

From 2011.igem.org

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=General PCR Protocol=
=General PCR Protocol=

Revision as of 21:45, 13 September 2011


General PCR Protocol

    1. Setup Amplification PCR Reaction
      1. 1uL Template
      2. 1uL 25mM dNTP's
      3. 10uL Phusion HF Buffer
      4. 0.5uL Forward Primer (Tm 65)
      5. 0.5uL Reverse Primer (Tm 65)
      6. 0.5uL Phusion polymerase
      7. 36.5uL diH2O
    2. Amplification PCR Reaction
      1. 98C - 30s
      2. 98C - 10s
      3. 63C - 10s
      4. 72C - 30s/kb target gene
      5. Repeat 2-4 29x
      6. 72C - 5min
      7. 10C - forever
Retrieved from "http://2011.igem.org/Team:Washington/Protocols/PCR"