Team:Washington/Protocols/Induc studies.
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Revision as of 22:31, 15 September 2011
Induction Studies of Gene-Protein Fusions (mamK-sfGFP and mamI-sfGFP)
- Obtain a tube and fill it with 5 mL TB + Amp + Chlor.
- Obtan another tube and fill it with 1 mL TB + Amp + Chlor.
- Starting with an already prepared PBS broth, pipet 20 uL into each tube.
- Add 200 uM IPTG into each tube and incubate at room temperature.
- Prepare a microscope slide of each sample and Image using a microscope.
- Pipet ~ 5 ul of each sample onto separate slides.
- Cover the samples with agarose while trying not to introduce any bubbles.