Team:Washington/Protocols/Colony

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Colony PCR with Green tag

Master mix(7ul):

1ul 10uM forword primer

1ul 10uM reverse Primer

5ul 2x Green tag

Cell water(3ul): Pick one colony from the plate and mix with 10ul of ddH2O to make 10ul of cell water

Reaction = Master mix(7ul) + Cell water(3ul) = 10ul per tube

Use program "Colony" & change the extention time (1min per kb)

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