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Team:Washington/Protocols/Colony
From 2011.igem.org
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{{Template:Team:Washington/Templates/Top}} | {{Template:Team:Washington/Templates/Top}} | ||
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=Colony PCR with Green tag= | =Colony PCR with Green tag= | ||
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Master mix(7ul): | Master mix(7ul): | ||
| - | 1ul 10uM | + | 1ul 10uM forward primer |
1ul 10uM reverse Primer | 1ul 10uM reverse Primer | ||
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Reaction = Master mix(7ul) + Cell water(3ul) = 10ul per tube | Reaction = Master mix(7ul) + Cell water(3ul) = 10ul per tube | ||
| - | Use program "Colony" & change the | + | Use program "Colony" & change the extension time (1 min per kb) |
Revision as of 01:44, 14 September 2011
Colony PCR with Green tag
Master mix(7ul):
1ul 10uM forward primer
1ul 10uM reverse Primer
5ul 2x Green tag
Cell water(3ul): Pick one colony from the plate and mix with 10ul of ddH2O to make 10ul of cell water
Reaction = Master mix(7ul) + Cell water(3ul) = 10ul per tube
Use program "Colony" & change the extension time (1 min per kb)
