Team:Washington/Parts

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Data Page




Data Summary

Data for Favorite New Parts

Diesel Production

1, 2. [http://partsregistry.org/Part:BBa_K590025 BBa_K590025: The PetroBrick] - A modular and open platform for the biological production of diesel fuel. The PetroBrick consists of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590032 AAR] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590031 ADC], each behind a standard Elowitz RBS. All of this is under regulation by a high constitutive promoter in pSB1C3.

Gluten Destruction

3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590023 BBa_K590023: KumamaMax]- A modified version of the enzyme Kumamolisin, a protease ofthe sedolisin family native to Alicyclobacillus sendaiensis known to be active at low pH and elevated temperatures. To Kumamolisin, the mutations N291D, G319S D358G, D368H increase activity to the PQLP peptide, an antigenic epitope in gliadin, 118-fold.

Gibson Assembly Toolkit

4. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590010 BBa_K590010: pGA1A3_pLacGFP], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590011 BBa_K590011: pGA1C3_pLacGFP], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590012 BBa_K590012: pGA4C5_pLacGFP], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590013 BBa_K590013: pGA4A5_pLacGFP], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590014 BBa_K590014: pGA3K3_pLacGFP] - These are plasmid backbones optimized for use in Gibson cloning, with a variety of copy numbers and antibiotic resistances.

Magnetosome Toolkit

5. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590015 BBa_K590015: sfGFP_mamK_pGA1C3] - This part consists of the mamK gene from Magnetospirillum magneticum strain AMB-1, fused to sfGFP, in the backbone of pGA1C3. MamK was previously reported to be required for proper alignment of magnetosomes in a chain in magnetic bacteria.
6. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K590016 BBa_K590016 sfGFP_mamI_pGA1C3] - This part consists of mamI gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. MamI is a membrane-localized protein that localizes magnetic vesicles to the surface of cells, thus forming characteristic magnetosome chains.

Data for Existing Parts

1. [http://partsregistry.org/Part:BBa_K314100:Experience K314100: High Constitutive Expression Cassette] (Washington, iGEM 2010) - We used this part to express our Petrobrick, and determined that 0.5 uM IPTG is sufficient to express our system.

Improved Parts

1. [http://partsregistry.org/Part:BBa_K590059 BBa_K590059], [http://partsregistry.org/Part:BBa_K590060 BBa_K590060], [http://partsregistry.org/Part:BBa_K590061 BBa_K590061: LuxC, D, and E] - Formerly parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K325902 BBa_K325902] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K325903 BBa_K325903]. luxC, D, and E were not separated or codon-optimized. We codon-optimized these genes and put them under the control of standard Elowitz RBS's. This was accomplished by the Alternate Aldehyde branch of the Alkane Production team.

All Submitted Parts

<groupparts>iGEM011 Washington</groupparts>