Team:WITS-CSIR SA

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                <img src="https://static.igem.org/mediawiki/2011/6/63/Wits_Logo_Biotweet_Rearrange.gif" alt="Biotweet" height="60px"
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                 Abstract            </div>
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E.coli has chemoreceptors and proteins which act downstream to regulate the bacterium's proportions of runs and tumbles resulting in its being stationary or moving respectively. The CheZ protein dephosphorylates the CheY protein, allowing the bacteria to run instead of tumbling. We used CheZ mutants, unable to move, and only allowed the expression of the CheZ protein on a riboswitch sensitive to theophylline. This allows the bacteria to be artificially sensitive to it. Once the attractant was reached, these genes were switched off and the bacteria was sensitive to another attractant, allowing it to return to its start position.</p>
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                 <div data-dojo-type="dijit.TitlePane" data-dojo-props="title:'Biotweet: A riboswitch-controlled location-based networking framework'">
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                  </a><a href="#">
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                 <br />
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                  <strong>This</strong> dude has some <em>chalk</em>. Mostly I don't have any content
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<div data-dojo-type="dijit.TitlePane" data-dojo-props="title:'What is Biotweet?' ">
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                  <a href="#">to put here</a>.
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<p>A defining characteristic of humanity is the constantly evolving sophistication with which human beings communicate and store information. Biologically, data communication networks exist too. The transfer of data occurs via signalling molecules incorporated into highly regulated and integrated networks.Our goal is to construct a bacterial communication network that will allow a group of engineered bacteria to transport messages in a directed manner, with the final aim of completing location-based functional tasks.We have focused on one aspect of this network: the directed transport of the message-carrying “communication modules” within our biological network. These communication modules will take the form of bacteria, which can move over physical distances carrying information. We have achieved this through engineering the exogenously controlled chemotactic behaviour of bacteria using synthetic riboswitches to regulate the expression of the CheZ protein required for bacterial motility.
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                  <strong>This</strong> is the same thing as the last...
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                  <strong>A board</strong> ... with writing.
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                  <strong>Reading </strong>something.
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<td valign="left"> <img src="https://static.igem.org/mediawiki/2011/8/8e/Frontpage_newbanner1.jpg" alt="biotweet" height="432" />
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                      Boomerang Bacteria                    </div>
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                      We have decided on a project manipulating bacterial chemotaxis. What is chemotaxis? Well, it's the ability of bacteria to swim towards something they're attracted to and swim away from chemicals that are potentially dangerous to them. We will be engineering bacteria to be attracted to a substance, reach the source of that substance and once they are there, turn off their attraction to this substance and become attracted to another substance. This means we can send a bacteria out to detect something. Once it's found its highest concentration, it will swim back to the start to report back. This project has potential applications in mining, medicine and water treatment!                </div>
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<strong>Project</strong>  
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<p class="slider-text">Biotweet is a biological networking framework. Our project page details all laboratory and modelling aspects of this project <a style="color:white;" href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Concept"><b>[Read more]</b></a></p>  
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                            Meet the team                         </div>
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                            The CSIR Wits South Africa team consists of six enthusiastic undergraduate students
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                  each having their own area of expertise. Four of the members are studying science
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                  and two are studying engineering at the University of the Witwatersrand. The biologists
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                  are from the schools of Molecular and Cell Biology and Molecular Medicine and Hematology.
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                  The team has two engineering students, one studying information engineering and
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                  the other, chemical engineering. This team is a dynamic one where each team member
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<p class="slider-text">Our team consists of five enthusiastic undergraduate students each having their own area of expertise. Four of the members are studying science
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                  has something unique to offer to the competition.
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              and one is studying engineering at the University of the Witwatersrand. <a style="color:white;" href="https://2011.igem.org/Team:WITS-CSIR_SA/AboutUs/MeetTheTeam"><b>[Read more]</b></a></p>
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                            Software                          </div>
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<strong>Parts submitted</strong>
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<p class="slider-text">We submitted 16 parts to the registry. We also characterized a number of parts, including two theophylline riboswitches! <a style="color:white;" href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Parts"><b>[Read more]</b></a></p>  
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                            We created the following software during the competition.                           </div>
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<strong>Achievements</strong>
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<p class="slider-text">The team is proud to be a prize winner and one of the finalists from the European jamboree! For more details of our achievements in this competition <a style="color:white;" href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Achievements"><b>[click here]</b>!</a>
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Latest revision as of 20:45, 28 October 2011

Media:Example.ogg<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Biotweet

Biotweet iGem

A defining characteristic of humanity is the constantly evolving sophistication with which human beings communicate and store information. Biologically, data communication networks exist too. The transfer of data occurs via signalling molecules incorporated into highly regulated and integrated networks.Our goal is to construct a bacterial communication network that will allow a group of engineered bacteria to transport messages in a directed manner, with the final aim of completing location-based functional tasks.We have focused on one aspect of this network: the directed transport of the message-carrying “communication modules” within our biological network. These communication modules will take the form of bacteria, which can move over physical distances carrying information. We have achieved this through engineering the exogenously controlled chemotactic behaviour of bacteria using synthetic riboswitches to regulate the expression of the CheZ protein required for bacterial motility.

biotweet biotweet


  • Project Image
    Project

    Biotweet is a biological networking framework. Our project page details all laboratory and modelling aspects of this project [Read more]

  • Meet the team

    Our team consists of five enthusiastic undergraduate students each having their own area of expertise. Four of the members are studying science and one is studying engineering at the University of the Witwatersrand. [Read more]

  • Example Slide Image 3
    Parts submitted

    We submitted 16 parts to the registry. We also characterized a number of parts, including two theophylline riboswitches! [Read more]

  • Example Slide Image 4
    Achievements

    The team is proud to be a prize winner and one of the finalists from the European jamboree! For more details of our achievements in this competition [click here]!



Retrieved from "http://2011.igem.org/Team:WITS-CSIR_SA"