Team:USC/Notebook/Week5

From 2011.igem.org

Revision as of 03:56, 29 September 2011 by Nolansardesai (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 5:

07/05/2011
1. digestion step by step for MET25 plasmid see protocols in general protocols


07/06/2011
1. Purify Gel DNA
2. Run a gell test :
MET25, pRS423-426, tetO+cFP, tetO+RFP, tetO+GFP,CAS3, CASO 3. PCR CASO primers


07/07/2011
1. Transformation for all 4 ligation plasmids:
TPS1, MSN2, ELO1, OLE1+pRS vectors
2. Gel verify for CASO
3. Amplify the verified products by PCR
4. Reisolate the genomic DNA
5. PCR CASO by using old primers