Team:USC/Notebook/Week2

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Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 2:

06/13/2011
1. Lab meeting

06/14/2011
1. Order the primer for PCR

06/15/2011
1. DNA isolation
2. PCR genes we need

06/16/2011
1. DNA isolation for E.Coli strains DH5 α, HT115, BL21
2. PCR

06/17/2011
1. Run a gel to test the genes we made yesterday
Observation: all genes for Yeast and CRISPR project worked
2. Removal of single stranded Extension For:
pSB1A3, pSb1K3, OLE1, MSN2, TSP1, CAS0