From 2011.igem.org
(Difference between revisions)
|
|
| Line 1: |
Line 1: |
| | {{OrganizationS&T}} | | {{OrganizationS&T}} |
| - | <div style="font-color:black; font-size:large; font-weight:bold">
| |
| - | <b>Notebook</b>
| |
| - | </div>
| |
| - |
| |
| - | <div id="box" style="width: 98%; margin-left: 0px; padding: 0px; border: 10px solid #006400; background-color: #c0c0c0;">
| |
| - | <div style="font-size: large; font-weight: bold" align="center">
| |
| - | July
| |
| - | </div>
| |
| - |
| |
| - | {| style="color:green; background-color: #c0c0c0" border="1px"
| |
| - | ! Sunday
| |
| - | ! Monday
| |
| - | ! Tuesday
| |
| - | ! Wednesday
| |
| - | ! Thursday
| |
| - | ! Friday
| |
| - | ! Saturday
| |
| - | |-
| |
| - | | 26
| |
| - | | 27
| |
| - | | 28
| |
| - | | 29
| |
| - | *Resuspension
| |
| - | of Omp-R,
| |
| - | eYFP, and RBS<br>
| |
| - | [[7/29 Details]]
| |
| - | | 30
| |
| - | | 1
| |
| - | | 2
| |
| - | |-
| |
| - | | 3
| |
| - | | 4
| |
| - | | 5
| |
| - | *Isolated
| |
| - | plasmids
| |
| - |
| |
| - | | 6
| |
| - | *Mini Preps.
| |
| - | *Digest1
| |
| - |
| |
| - | | 7
| |
| - | *Ran Gel for
| |
| - | Digest1
| |
| - | (Gel Incon-
| |
| - | clusive)
| |
| - | | 8
| |
| - | *Re-did Digest1
| |
| - | and Gel1
| |
| - | *Cut out bands
| |
| - | | 9
| |
| - | |-
| |
| - | | 10
| |
| - | | 11
| |
| - | *Ectracted
| |
| - | plasmid parts
| |
| - | from gel
| |
| - | *Ligation1
| |
| - | | 12
| |
| - | *Transformation
| |
| - | of E.coli with
| |
| - | lig.1 products
| |
| - | *Digest2 (eYFP
| |
| - | and RBS)
| |
| - | *Gel of Digest2
| |
| - | *Extracted bands
| |
| - | of Digest2
| |
| - | | 13
| |
| - | *Transform.1
| |
| - | had colonies
| |
| - | *Re-did eYFP
| |
| - | digest2
| |
| - | *Ran eYFP D2 gel
| |
| - | *Extracted eYFP
| |
| - | *Ligation2
| |
| - | | 14
| |
| - | *Transformation2
| |
| - | *Innoculated
| |
| - | transform.1 into
| |
| - | broth
| |
| - | | 15
| |
| - | *Isolated plasmids
| |
| - | from transform.1
| |
| - | | 16
| |
| - | |-
| |
| - | | 17
| |
| - | | 18
| |
| - | | 19
| |
| - | | 20
| |
| - | | 21
| |
| - | | 22
| |
| - | | 23
| |
| - | |-
| |
| - | | 24
| |
| - | | 25
| |
| - | | 26
| |
| - | | 27
| |
| - | | 28
| |
| - | | 29
| |
| - | | 30
| |
| - | |}
| |
| - |
| |
| - | </div>
| |
Revision as of 09:40, 26 September 2011
"
