Team:Kyoto

From 2011.igem.org

Summary

We create new E.coli which hunt and eat insects. it is named Carnivorous E.coli. Carnivorous E.coli emits light when hungry, and insects come near it attracted by the light. Then, it secretes viscous material to catch the insects. The insects caught are solved by protease and chitinase that Carnivorous E.coli secretes also. So, Carnivorous E.coli can hunt!

Project Hunger

It is a burden for the E.coli to emit light. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough.

Project Luminescence

There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.

Project Digestion

An insect body is covered with a hard integument containing chitin mainly. To decompose the integument, we used ChiA genes, which encode chitinase. In order to measure the chitinase activity of culture supernatant, we evaluated the effect of medium and cell growth.