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Team:Kyoto
From 2011.igem.org
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== <html><a href="https://2011.igem.org/Team:Kyoto/Hunger"><span class="red_title">Project Hunger</span></a></html> == | == <html><a href="https://2011.igem.org/Team:Kyoto/Hunger"><span class="red_title">Project Hunger</span></a></html> == | ||
| + | <html><div id="hunger"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></div></html> | ||
It is a burden for the E.coli to emit light. | It is a burden for the E.coli to emit light. | ||
This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough. | This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough. | ||
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== <html><a href="https://2011.igem.org/Team:Kyoto/Luminescence"><span class="green_title">Project Luminescence</span></a></html> == | == <html><a href="https://2011.igem.org/Team:Kyoto/Luminescence"><span class="green_title">Project Luminescence</span></a></html> == | ||
| + | <html><div id="capture"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></div></html> | ||
There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. | There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. | ||
Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from | Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from | ||
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== <html><a href="https://2011.igem.org/Team:Kyoto/Digestion"><span class="blue_title">Project Digestion</span></a></html> == | == <html><a href="https://2011.igem.org/Team:Kyoto/Digestion"><span class="blue_title">Project Digestion</span></a></html> == | ||
| + | <html><div id="digestion"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></div></html> | ||
In this part, we aimed to create ''E.coli'' which digest bugs and survive under the nitrogen-poor condition. So, we decided to use SAM-P20 and ChiA genes, which encode protease and chitinase. In order to measure the activity of each enzyme correctly, we examined assay methods first. | In this part, we aimed to create ''E.coli'' which digest bugs and survive under the nitrogen-poor condition. So, we decided to use SAM-P20 and ChiA genes, which encode protease and chitinase. In order to measure the activity of each enzyme correctly, we examined assay methods first. | ||
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| + | #hunger, #capture, #digestion { | ||
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| + | #hunger img {margin-left: -2px;} | ||
| + | #capture img {margin-left: -254px;} | ||
| + | #digestion img {margin-left: -506px;} | ||
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Revision as of 15:04, 3 October 2011
Contents |
Summary
We create new E.coli which hunt and eat insects. it is named Carnivorous E.coli. Carnivorous E.coli emits light when hungry, and insects come near it attracted by the light. Then, it secretes viscous material to catch the insects. The insects caught are solved by protease and chitinase that Carnivorous E.coli secretes also. So, Carnivorous E.coli can hunt!
Project Hunger
Project Luminescence
Project Digestion
