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| | ======Yeast Toolkit Background====== | | ======Yeast Toolkit Background====== |
| - | As part of this project, we are developing a yeast expression platform including a library of promoters, termination sequences, and shuttle vectors. While iGEM has many well-characterized basic parts for ''E. coli'', yeast remains a largely untapped resource. The inclusion of shuttle vectors in our platform will allow future genetic engineers to take advantage of ''E. coli'''s ability to rapidly replicate while still being able to deploy their construct in ''S. cerevisiae''. We hope our work will facilitate the use of yeast in future iGEM projects.
| + | We are developing a fully BioBrick compatible yeast expression platform including a library of promoters, termination sequences, and shuttle vectors. While iGEM has many well-characterized basic parts for ''E. coli'', yeast remains a largely untapped resource. The inclusion of shuttle vectors in our platform will allow future genetic engineers to take advantage of both ''E. coli'''s ability to rapidly replicate and ''S. cerevisiae'''s production capabilities. We also aim to add the Violacein pathway to yeast as a reporter, along with the pigments in the beta-Carotene pathway. It will be the first time the violacein pathway will be expressed in a eukaryotic organism. We hope our promoters, 3'UTRs, vectors, and reporters will facilitate the use of yeast in future iGEM projects. |
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| - | We aim to add the Violacein pathway to yeast using yeast promoters and 3'UTR's. It will be the first time the violacein pathway will be expressed in a eukaryotic organism. For further details on the purple pigment violacein, please visit the [https://2009.igem.org/Team:Cambridge/Project/VI01 "Violacein Page"] on the Cambridge iGEM 2009 website.
| + | For further details on the purple pigment violacein, please visit the [https://2009.igem.org/Team:Cambridge/Project/VI01 "Violacein Page"] on the Cambridge iGEM 2009 website. |
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Yeast Toolkit Background
We are developing a fully BioBrick compatible yeast expression platform including a library of promoters, termination sequences, and shuttle vectors. While iGEM has many well-characterized basic parts for E. coli, yeast remains a largely untapped resource. The inclusion of shuttle vectors in our platform will allow future genetic engineers to take advantage of both E. colis ability to rapidly replicate and S. cerevisiaes production capabilities. We also aim to add the Violacein pathway to yeast as a reporter, along with the pigments in the beta-Carotene pathway. It will be the first time the violacein pathway will be expressed in a eukaryotic organism. We hope our promoters, 3'UTRs, vectors, and reporters will facilitate the use of yeast in future iGEM projects.
For further details on the purple pigment violacein, please visit the "Violacein Page" on the Cambridge iGEM 2009 website.
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