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| | ======Promoters and UTRs====== | | ======Promoters and UTRs====== |
| - | As we began our work with ''Saccharomyces cerevisiae'', it came to our attention that the database is deficient in genes for this organism. This deficiency is due in part to the fact that ''E. coli'' genes are widespread through the database, whereas yeast remains a largely untapped area. In order to address this problem and facilitate the use of yeast in synthetic biology we have decided to provide the basic parts required for gene use and assembly. We constructed some basic parts including promoters and termination sequences of varying strengths. This will allow future teams the ease of use of premade biobricked sequences to use with their genes.
| + | The iGEM registry is lacking in promoter and terminators sequences for yeast, especially when compared with the number available for use in ''E. coli''. In order to address this problem and increase the use of yeast in synthetic biology we have decided to provide a library of promoter and terminator sequences from 12 yeast genes: 4 causing high expression, 4 causing medium expression, and 4 causing low expression. We hope these BioBrick compatible parts will make it easier for future iGEM teams to work with ''Saccharomyces cerevisiae''. |
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| | ======Promoters====== | | ======Promoters====== |
Promoters and UTRs
The iGEM registry is lacking in promoter and terminators sequences for yeast, especially when compared with the number available for use in E. coli. In order to address this problem and increase the use of yeast in synthetic biology we have decided to provide a library of promoter and terminator sequences from 12 yeast genes: 4 causing high expression, 4 causing medium expression, and 4 causing low expression. We hope these BioBrick compatible parts will make it easier for future iGEM teams to work with Saccharomyces cerevisiae.
Promoters
Yeast promoter sequences of varying strength have been provided for our work. The following are the available promoters:
UTRs
Similarly, the following UTRs, or termination sequences have been provided for our work:
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