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Team:Imperial College London/test9
From 2011.igem.org
fjawelkfjlewa gf agre gaergaerg jrjg roiajg eaorijg aoirejg reoajg areiojg arojf aiowrej waroijf iaweojf oweajf iowajefio wjaeiof jiowej fiowef
fjawelkfjlewa gf agre gaergaerg jrjg roiajg eaorijg aoirejg reoajg areiojg arojf aiowrej waroijf iaweojf oweajf iowajefio wjaeiof jiowej fiowef
fjawelkfjlewa gf agre gaergaerg jrjg roiajg eaorijg aoirejg reoajg areiojg arojf aiowrej waroijf iaweojf oweajf iowajefio wjaeiof jiowej fiowef
fjawelkfjlewa gf agre gaergaerg jrjg roiajg eaorijg aoirejg reoajg areiojg arojf aiowrej waroijf iaweojf oweajf iowajefio wjaeiof jiowej fiowef
wfeaw CPEC fawef eawf afaw wea
CPEC (Circular Polymerase Extension Cloning) is a primer-independent PCR assembly technique which relies on overlaping sequences between each part to be assembled. With a denaturing step, the double stranded DNA is melted, allowing compatible single stranded ends of each part to joined. For this reason it is essential that the parts are designed with homologous ends (the fragments we used were designed with 60 bp overlaps). The annealed overlapping ends then serve as primers for polymerase extension to join the parts into a seamless construct.
