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Team:Imperial College London/Protocols General
From 2011.igem.org
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| + | <h2>CPEC assembly</h2> | ||
| + | <p>Make up 50 ul reactions with 5 ul DNA total with each part to be assembled at equimolar concentrations (ie. make appropriate dilutions)</p> | ||
| + | <p>-33.5 ul ddH2O</p> | ||
| + | <p>-10 ul Phusion HF buffer (5x)</p> | ||
| + | <p>-1 ul dNTP mix (40 mM)</p> | ||
| + | <p>-5 ul DNA (1-50 ng)</p> | ||
| + | <p>-0.5 Phusion high fidelity DNA polymerase (2 U/ul<sup>-1</sup>)</p> | ||
| + | |||
<h2>Heat Stability of GFP</h2> | <h2>Heat Stability of GFP</h2> | ||
<hr style="color:#225323;"/> | <hr style="color:#225323;"/> | ||
Revision as of 16:19, 15 September 2011
Protocols
This page lists all the protocols used in our project. We have classified them into five main categories as follow.
General
Cell Transformation
DNA Rehydration
Colony PCR
CPEC
CPEC assembly
Make up 50 ul reactions with 5 ul DNA total with each part to be assembled at equimolar concentrations (ie. make appropriate dilutions)
-33.5 ul ddH2O
-10 ul Phusion HF buffer (5x)
-1 ul dNTP mix (40 mM)
-5 ul DNA (1-50 ng)
-0.5 Phusion high fidelity DNA polymerase (2 U/ul-1)
