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Team:Grenoble/Projet/Biobricks
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| - | + | <h1>Biobricks</h1> | |
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| - | + | <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Grenoble">Here</a> you can find all our news and composite parts we send to the registry. | |
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Revision as of 23:55, 28 October 2011
Si vous éditez la page commencez par décommenter ces lignes, publier, PUIS commencer à faire ce que vous avez à faire et quand vous avez fini de publier remettez en commentaire.
Ne laissez pas le carré trop longtemps si vous n'éditez pas, chaque fois reprenez ce qui est sur internet plutôt que ce que vous avez sur votre PC
Biobricks
Here you can find all our news and composite parts we send to the registry.
<groupparts>iGEM011 Grenoble</groupparts>
- You can also find in the registry some of our intermediate constructions :
| BBa_S04602 | RBS-CinR, BBa_B0034+BBa_C0077 |
| BBa_S04603 | RBS-TetR, BBa_B0034+BBa_C0040 |
| BBa_S04606 | RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077 |
| BBa_S04605 | RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240 |
| BBa_S04607 | pLac-CinR, BBa_R0011+BBa_C0077 |
| BBa_S04618 | pLac-CinR, BBa_K545006+BBa_I13401 |
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MerR-del (K545103)MerR (BBa_K346001) without RBS PCR gel Well 1: Positive control (pSB4K5) Well 2: Negative control (Without DNA) Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb) Confirmed by sequencing |
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pMerT-GFP (K545002)Original parts: K346002, E0240 PCR gel Well 1: MW Well 2: empty well Well 3 and 4: clonning failure Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb) Confirmed by sequencing |
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pCin-CrtEBI (K545000)Original parts: R0077, K274100 PCR gel Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb) Well 4: MW Confirmed by sequencing |
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pConst-RBS-CinR (K545004)Original parts: J23119, B0034 C0077 Restriction gel Wells 1 and 2: pConst-CinR(expected at 950pb) Wells 4 and 5: cloning failure Wells 3 and 6: empty wells plasmids were obtained for each well at the expected size,about 2000pb. Confirmed by sequencing |
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pLac-CinR (S04607)Original parts: R0011, E0240 Restriction gel pConst-GFP (K545001)Original parts: J23119, E0240 Restriction gel Well 1: pLac-CinR (expected at 950pb) Well 2: pConst-CinR (expected at 900pb) plasmids were obtained for each well at the expected size,about 2000pb. Confirmed by sequencing |
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RBS-CinR (S04602)Original parts: B0034, C0077 PCR gel Well 1: RBS-CinR (expected at 1000) Wells 2, 3 and 4: clonning failure Confirmed by sequencing |
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RBS-TetR (S04603)Original parts: B0034, C0040 PCR gel well 1: RBS-TetR (expected at 950pb) Confirmed by sequencing |
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