Team:Grenoble/Projet/Biobricks

From 2011.igem.org

Grenoble 2011, Mercuro-Coli iGEM


Biobricks

Here you can find all our news and composite parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts>

You can also find in the registry some of our intermediate constructions:

BBa_S04602

RBS-CinR, BBa_B0034+BBa_C0077

BBa_S04603

RBS-TetR, BBa_B0034+BBa_C0040

BBa_S04606

RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077

BBa_S04605

RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240

BBa_S04607

pLac-CinR, BBa_R0011+BBa_C0077

BBa_S04618

pLac-CinR, BBa_K545006+BBa_I13401

MerR-del (K545103)


MerR (BBa_K346001) without RBS
PCR gel

Well 1: Positive control (pSB4K5)
Well 2: Negative control (Without DNA)
Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb)

Confirmed by sequencing

pMerT-GFP (K545002)


Original parts: K346002, E0240
PCR gel

Well 1: MW
Well 2: empty well
Well 3 and 4: clonning failure
Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb)

Confirmed by sequencing

pCin-CrtEBI (K545000)


Original parts: R0077, K274100
PCR gel

Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb)
Well 4: MW

Confirmed by sequencing

pConst-RBS-CinR (K545004)


Original parts: J23119, B0034 C0077
Restriction gel

Wells 1 and 2: pConst-CinR(expected at 950pb)
Wells 4 and 5: cloning failure
Wells 3 and 6: empty wells
plasmids were obtained for each well at the expected size,about 2000pb.

Confirmed by sequencing

pLac-CinR (S04607)


Original parts: R0011, E0240
Restriction gel

pConst-GFP (K545001)


Original parts: J23119, E0240
Restriction gel

Well 1: pLac-CinR (expected at 950pb)
Well 2: pConst-CinR (expected at 900pb)
plasmids were obtained for each well at the expected size,about 2000pb.

Confirmed by sequencing

RBS-CinR (S04602)


Original parts: B0034, C0077
PCR gel

Well 1: RBS-CinR (expected at 1000)
Wells 2, 3 and 4: clonning failure

Confirmed by sequencing

RBS-TetR (S04603)


Original parts: B0034, C0040
PCR gel

well 1: RBS-TetR (expected at 950pb)

Confirmed by sequencing