Team:Freiburg/Notebook/13 July

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Waiting for sequencing results of parts BBa_15008 and BBa_15009.
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{{:Team:Freiburg/Templates/header}}
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/12_July">Previous entry</a>
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<div id="notebook-title">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 13 July </a>
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<div id="notebook-next">
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/14_July">Next entry</a>
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==<span style="color:red;">red light receptor</span>==
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'''Investigators: Julia'''
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Waiting for sequencing results of parts BBa_I15008 and BBa_I15009.
Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3.
Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3.
She also did a test digest to see if the insert is in the vector.
She also did a test digest to see if the insert is in the vector.
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===Miniprep===
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'''Investigators: Jakob'''
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Miniprep of cph8 from M30109 in pSB1T3
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DNA concentration was measured with NanoDrop:
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{| cellpadding="10" cellspacing="0" border="1"
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|samples
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|DNA concentration
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|260/280
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|260/230
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|-
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|cph8a
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|33.7
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|1.79
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|1.74
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|-
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|cph8b
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|41.2
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|1.79
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|1.39
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|-
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|cph8c
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|24.1
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|1.87
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|1.47
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|-
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|cph8d
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|30
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|1.74
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|1.84
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|-
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|cph8e
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|34
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|1.81
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|1.86
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|-
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|cph8f
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|30
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|1.83
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|1.81
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|}
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===Testdigest===
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'''Investigators: Julia'''
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Testdigest of cph8
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*Enzyme 1: EcoRI
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*Enzyme 2: PstI
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<br/>
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<br/>
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===Transformation===
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'''Investigators: Julia'''
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Transformation of BBa_I15010 (in the vector pSB2K3) in cells. Sophie plated out the cells.
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<span style="color:blue;">Comments: No colonies grew on the plates.</span>
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===PCR===
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'''Investigators: Julia'''
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New PCR for red light receptor.
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Primer:
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*P10
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*P11
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DNA template:
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*M30109
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<span style="color:blue;">Comments: There were no bands on the gel.</span>
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<br/>
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<br/>
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==<span style="color:grey;">Precipitator</span>==
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===Miniprep===
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'''Investigators: Sophie, Sandra'''
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<span style="color:blue;">Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.</span>
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Miniprep of the plastik-binding domain: There were always picked two colonies of the same plate (sample and sample*).
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{| cellpadding="10" cellspacing="0" border="1"
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|sample / sample*
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|DNA concentration
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|280/230
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|260/230
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|-
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|M2a alpha / M2a* alpha
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|66.5 / 91.6
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|1.84 / 1.75
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|1.76 / 1.40
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|-
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|M2a beta / M2a* beta
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|83.8 / 13.8
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|1.80 / 1.72
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|1.52 / 0.97
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|-
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|M2a gamma / M2a* gamma
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|118.9 / 56.1
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|1.68 / 1.86
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|1.00 / 2.13
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|-
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|M2a delta / M2a* delta
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|101.5 / 66.0
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|1.74 / 1.85
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|1.26 / 2.16
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|-
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|M2a epsilon / M2a* epsilon
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|112.9 / 69.9
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|1.68 / 1.82
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|1.00 / 1.82
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|-
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|M2b alpha / M2a* alpha
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|79.7 / 74.9
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|1.87 / 1.83
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|2.20 / 2.14
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|-
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|M2b Beta/M2b* beta
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|122.3/74.4
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|1.71/1.84
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|1.04/2.18
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|-
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|M2b gamma / M2b* gamma
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|111.2 / 72.0
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|1.70 / 1.83
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|1.08 / 1.99
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|-
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|M2b epsilon / M2b* epsilon
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|88.5 / 90.09
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|1.72 / 1.79
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|1.19 / 1.78
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|-
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|M3 alpha / M3* alpha
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|136.3 / 93.9
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|1.71 / 1.78
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|1.10 / 1.74
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|-
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|M3 beta / M3* beta
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|54.7 / 79.2
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|1.91 / 1.83
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|2.03 / 2.13
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|-
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|M3 gamma / M3* gamma
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|130.6 / 141.7
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|1.66 / 1.80
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|0.87 / 1.88
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|-
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|M3 delta / M3* delta
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|79.6 / 82.9
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|1.76 / 1.85
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|1.27 / 2.19
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|-
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|M3 epsilon / M3* epsilon
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|84.1 / 122.5
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|1.79 / 1.69
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|1.45 / 1.03
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|}
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<br/>
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<br/>
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===Testdigest===
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'''Investigators: Sophie'''
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The minipreps were digested and loaded onto a gel.
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Latest revision as of 00:55, 22 September 2011


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