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MITOMI

Mitomi is a high troughput microfluidic system designed to mesure low affinity molecular interactions by mechanically trapping them.

Purpose: We applied this technology during our project for characterizing transcription factor binding energy landscapes in absolute terms by determining dissociation constants (Kd) for a comprehensive set of target DNA sequences.

Schematic of the Mitomi platform:

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Advantages: The movable memrane shown in the scheme below enables measurements of transient interactions, exhibiting sub micromolar affinities which are generally problematic.