"
Team:EPF-Lausanne/Tools/MITOMI
From 2011.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
replace with sth relevant 4us | replace with sth relevant 4us | ||
| + | |||
| + | '''Advantages:''' The movable memrane shown in the scheme below enables measurements of transient interactions, exhibiting sub micromolar affinities which are generally problematic. | ||
[[File:Mitomi button.PNG]] | [[File:Mitomi button.PNG]] | ||
Revision as of 20:25, 7 August 2011
MITOMI
Mitomi is a high troughput microfluidic system designed to mesure low affinity molecular interactions by mechanically trapping them.
Purpose: We applied this technology during our project for characterizing transcription factor binding energy landscapes in absolute terms by determining dissociation constants (Kd) for a comprehensive set of target DNA sequences.
Schematic of the Mitomi platform:
replace with sth relevant 4us
Advantages: The movable memrane shown in the scheme below enables measurements of transient interactions, exhibiting sub micromolar affinities which are generally problematic.
