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Team:EPF-Lausanne/Tools/MITOMI
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[[File:Mitomi button.PNG]] | [[File:Mitomi button.PNG]] | ||
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| + | ==Running a MITOMI experiment== | ||
[[File:EPFL2011_MITOMI_run_illustration.png|700px]] | [[File:EPFL2011_MITOMI_run_illustration.png|700px]] | ||
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| + | ==MITOMI scans explanation== | ||
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} | ||
Revision as of 06:49, 21 September 2011
MITOMI
Mitomi is a high troughput microfluidic system designed to mesure low affinity molecular interactions by mechanically trapping them.
Purpose: We applied this technology during our project for characterizing transcription factor binding energy landscapes in absolute terms by determining dissociation constants (Kd) for a comprehensive set of target DNA sequences.
Schematic of the Mitomi platform:
The control inputs control the valves that close the Detailed view of the chambers and of the movable membrane used for mechanical trapping, referred to as the 'Button' .
Advantages: The movable memrane shown in the scheme below enables measurements of transient interactions, exhibiting sub micromolar affinities which are generally problematic.
Running a MITOMI experiment
MITOMI scans explanation
