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Team:EPF-Lausanne/Our Project/TetR mutants/Conclusion
From 2011.igem.org
In Vivo & In Vitro Outline
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline
For some of the TetR mutants, we managed to have both an in vitro and in vivo characterization. The results are available in the table below.
| Mutant | Biobrick number | Affinity compared to WT - in vivo | Affinity compared to WT - MITOMI | ATC repression |
|---|---|---|---|---|
| V36F | BBa_K613013 | same | higher | altered |
| V36F W43S | BBa_K613014 | same | not tested | altered |
| E37A W43S T141A | BBa_K613015 | same | altered | altered |
| P39K | BBa_K613016 | no affinity | no affinity | normal |
| Y42F K108E | BBa_K613018 | reduced | not tested | normal |
| P39Q Y42M | BBa_K613019 | no affinity | not tested | normal |
The MITOMI results can be found here. The in vivo characterizations are on this page.
