Team:EPF-Lausanne/Our Project/Reporter Systems/tetR

From 2011.igem.org

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''ATC induction''
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====''ATC induction''====
To validate our first readout system, we performed platereader experiments with different concentrations of anhydrotetracycline (ATC). This molecule binds to the TetR dimers and induces conformational changes that make the transcription factor unable to bind to Ptet. As a result, RFP can be expressed in the cell, as shown in the cartoons below:
To validate our first readout system, we performed platereader experiments with different concentrations of anhydrotetracycline (ATC). This molecule binds to the TetR dimers and induces conformational changes that make the transcription factor unable to bind to Ptet. As a result, RFP can be expressed in the cell, as shown in the cartoons below:
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''Dose-response''
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====''Dose-response''====
[[File:EPFL_Nadine-exp3-doseresponse.png|600px]]
[[File:EPFL_Nadine-exp3-doseresponse.png|600px]]
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''Individual results''
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====''Mutants individual results''====
For each TetR mutant tested, the individual ''in vivo'' characterization results are displayed on their respective Registry page:
For each TetR mutant tested, the individual ''in vivo'' characterization results are displayed on their respective Registry page:
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''Mutants comparison''
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====''Mutants comparison''====
In order to see better the differences between different mutants, we made two graphs: one showing the induction curves of all six mutants without ATC and one showing all six mutants with the maximal ATC concentration (2000 ng/mL).
In order to see better the differences between different mutants, we made two graphs: one showing the induction curves of all six mutants without ATC and one showing all six mutants with the maximal ATC concentration (2000 ng/mL).
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'' Comparison with in vitro data''
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===='' Comparison with in vitro data''====
A table summing up the results obtained for the TetR mutants, both ''in vitro'' and ''in vivo'', is available [https://2011.igem.org/Team:EPF-Lausanne/Our_Project/TetR_mutants/Conclusion here].
A table summing up the results obtained for the TetR mutants, both ''in vitro'' and ''in vivo'', is available [https://2011.igem.org/Team:EPF-Lausanne/Our_Project/TetR_mutants/Conclusion here].

Revision as of 20:04, 25 October 2011