Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
Data
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Contents |
Favourite Parts
These are officially our three favourite parts:
Other New parts
T7 Promoter
Our lysis selection device uses what we refer to as the wild-type T7 Promoter:
In addition, we submitted the following mutants:
Variants without additional lac operator (constitutive):
Variants with additional lac operator (LacI repressed):
TetR mutants
In-vivo testing was used to characterise these:
- V36F mutant: K613013
- V36F W43S double mutant: K613014
- E37A W43S T141A triple mutant: K613015
- P39K mutant: K613016
- Y42F K108E double mutant: K613018
- P39Q Y42M double mutant: K613019
Medium-strength Plac
T7 promoter variants
Pre-existing parts we improved
- Experience - The Berkeley Lysis Device, BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments.