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Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
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we must have 3 favourite, then the others come in a "other biobrick" section | we must have 3 favourite, then the others come in a "other biobrick" section | ||
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| + | Our lysis selection device uses what we refer to as the wild-type T7 Promoter: | ||
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| + | [http://partsregistry.org/Part:BBa_K613001 K613001] | ||
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| + | In addition, we submitted the following mutants: | ||
Variants without additional lac operator (constitutive): | Variants without additional lac operator (constitutive): | ||
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=== T7 promoter variants === | === T7 promoter variants === | ||
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== Pre-existing parts we improved == | == Pre-existing parts we improved == | ||
Revision as of 01:37, 22 September 2011
Data
team, please check this page: [1]
Contents |
New parts
we must have 3 favourite, then the others come in a "other biobrick" section
Our lysis selection device uses what we refer to as the wild-type T7 Promoter:
In addition, we submitted the following mutants:
Variants without additional lac operator (constitutive):
Variants with additional lac operator (LacI repressed):
Medium-strength Plac
TetR Mutants
| TetR variants | Mutagenesis | Promoter | Cterminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
|---|---|---|---|---|---|---|---|---|---|
| E37AW43ST141A | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v |
| P39K | PCR-induced | T7 | His-tag | template | ITT tested, worked | v | BBa_K613016 | v | v |
| Y42F | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v |
| Y42FK108E | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v |
T7 promoter variants
Pre-existing parts we improved
- Experience - The Berkeley Lysis Device, BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments.
