Team:EPF-Lausanne/Notebook/September2011

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Nadine ligated the Plac biobrick into pSB1C3 and transformed. She also purified the TetR genes with Gibson extensions, sending them for sequencing after having done a new Gibson reaction. The Gibson reactions were also transformed, hoping that this time it will work.
Nadine ligated the Plac biobrick into pSB1C3 and transformed. She also purified the TetR genes with Gibson extensions, sending them for sequencing after having done a new Gibson reaction. The Gibson reactions were also transformed, hoping that this time it will work.
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== Saturday, 17 September 2011 ==
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Nadine made colony PCRs on the Gibson assemblies with TetR mutants and on Plac-biobrick ligations. For the Plac-biobrick, all the 5 colonies tested seem to have the Plac insert.
Nadine made colony PCRs on the Gibson assemblies with TetR mutants and on Plac-biobrick ligations. For the Plac-biobrick, all the 5 colonies tested seem to have the Plac insert.
Doug made all the graphs of Nadine's platereader experiments. We still have some platereaders left to do: J61002 Ptet-RFP wil be done tomorrow and hopefully the TetR mutants will be ready and co-transformed on Tuesday.
Doug made all the graphs of Nadine's platereader experiments. We still have some platereaders left to do: J61002 Ptet-RFP wil be done tomorrow and hopefully the TetR mutants will be ready and co-transformed on Tuesday.
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== Saturday, 17 September 2011 ==
 
{{:Team:EPF-Lausanne/Templates/Footer|title=Notebook: September 2011}}
{{:Team:EPF-Lausanne/Templates/Footer|title=Notebook: September 2011}}

Revision as of 15:07, 17 September 2011