Team:EPF-Lausanne/Notebook/July2011

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(Difference between revisions)
(Tuesday, 26 July 2011)
(Tuesday, 26 July 2011)
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A gel was run for the gradient PCR, that used HiFi enzyme. It gave no product! In conclusion, we shall wait for the High Fidelity PLUS Enzyme, which should get here tomorrow.
A gel was run for the gradient PCR, that used HiFi enzyme. It gave no product! In conclusion, we shall wait for the High Fidelity PLUS Enzyme, which should get here tomorrow.
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In the mean time, Doug ran an identical gradient PCR with Matt's HiFiPLUS enzyme, changing the buffer concentration to 10 ul per 50 ul and lengthening the extension step to 2'30'', as recommended by the datasheet.
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In the mean time, Doug ran an identical gradient PCR with Matt's HiFiPLUS enzyme, changing the buffer concentration to 10 ul per 50 ul and lengthening the extension step to 2'30", as recommended by the datasheet.
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Revision as of 14:39, 26 July 2011