The problem

In bioengineering gene expression is often regulated by inducible promoters. A useful method with a number of limitations. There exists only a few good inducible promoters. Most promoters are leaky, genes are still expressed in the off state. And most importantly laborious chromosomal engineering is needed to place a promoter in front of natural genes.

Gene silencing in bacteria is fundamental to basic and applied research. Gene knockouts are widely used to investigate gene function, but also have severe limitations. Gene knockout requires biotechnology which is only developed for a handful of organisms. Following the explosion of known bacterial species new paradigms for research on genes are needed. In addition it is extremely difficult to investigate essential genes using knockout methods.

The idea

Gene silencing and induction could be improved considerable by exploiting the chitobiose system found in E. coli. The system provides an engineering framework for gene silencing by utilizing trans-acting RNA regulation. Regulation of the chitobiose system contains unique and very interesting features. A small RNA regulates expression analogously to the highly versatile miRNAs of eukaryotes. The RNA selectively targets and facilitates the degradation of the mRNA of a target gene. The region used for targeting is easily identified upon inspection of the small RNA sequence, as it is highly similar to the targeted sequence. This enables sequence based flexibility and could be used as a universal tool for easy and specific gene silencing.

Providing sequence based flexibility it is possibly a universal tool for easy and specific gene silencing.

As an added bonus this system would work in addition to any previously employed promoter based regulation, and due to the existence of a second small RNA to regulate the first, highly advanced schemes of regulation such as pulses can be achieved.

In short we believe this to be a "Swiss army knife" of gene silencing.

The advantages

There are many reasons for using our system instead of, or in addition to, the more commonly used solutions. Here are a few:

The trap-RNA system provides unique flexibility for gene silencing in prokaryotes enabling control and tuning of gene expression. The specificity of the system depends on base pair complementarity. Therefore it can be designed to target any gene of interest by simply altering the sequences to match the target gene. Furthermore multiple trap-RNA systems can applied to the same biological circuit without interfering. Implementing sRNA and the trap-RNA into biological constructs they can be introduced by constitutive promoters or inducible promoters.

The biggest reason is ease of use: Design the system in silico, order it and transform it. Done... You have now knocked down a gene.

This can ease engineering of other systems. Picked a promoter that is too strong? Use our system, and only induce the small RNA a little, thus lowering the amount of mRNA without knocking it completely out. Thus you can fine-tune a system that has already been designed, or you can just design your system with the strongest promoter there is, and use our system to tune it.

Since the small RNA (as indicated by the name) is small, this is not a big expense.

The second big reason is area of applicability. Any organism that is:

• Sequenced
• Has known stable plasmids
• Has hfq
• Is transformable

Is a potential target for our system. This includes many organisms that are difficult or impossible to chromosomally engineer.

Within the controlled environment of Biobricks the possibilities become even greater. Design your target gene with a unique Shine-Dalgarno and you can target just that gene, or use the same Shine-Dalgarno for all you genes and turn off your entire construct at once.

For the scientists there are a few more advantages: You can easily knock down genes in the middle of a gene, you can temporarily knock down essential genes and transcription factors. And all of this without changing the chromosome, your strain remains wild-type up until the moment you knock down the targeted gene.

Two Examples