Team:Calgary/Notebook/Protocols/Process26

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<p>For the electrodes we used the Screen printed Carbon Electrodes from Pine Instruments. These electrodes needed to be plated in zinc phthalocyanine for our purposes.</p><br>
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<p>The electrode and the sample to be measured need to be in a solution that is conductive to electricity to be able to measure the oxidation. To make our buffer, we used calcium chloride dissolved in nanopure water the most effective concentration was found to be at 0.1M. Our solution was made according to the following:<p><br>
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<li>22.2g of CaCl<sub>2</sub></li>
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<p>The buffer should be made fresh for each sample that is to be run, with the total volume equalling 20mL in a 50mL beaker. The way to make the buffer is as follows:</p><br>
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<li>Dissolve 100µg of zinc phthalocyanine in 10mL of DMF.</li>
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<li>Add 1mL of the NaCl</li>
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<li>Stir until dissolved.</li>
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<li>Add 1mL of the NaH<sub>2</sub>PO<sub>4</sub></li>
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<li>Dip only the working electrode (outer electrode) into the solution, and hold it there for 5 seconds.</li>
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<li>Add (18-X)mL of nanopure water, where X is the amount in mL of sample to be measured (This brings the pH to about 8.6)</li></ol><br><br><br>
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<li>Let the electrode air dry until there is no more solution present on the electrode.</li>
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<p><b>NOTE</b>: This should be performed in a fume hood due to the toxic nature of DMF</p>
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Latest revision as of 02:16, 29 October 2011


Buffer Preparation

The electrode and the sample to be measured need to be in a solution that is conductive to electricity to be able to measure the oxidation. To make our buffer, we used calcium chloride dissolved in nanopure water the most effective concentration was found to be at 0.1M. Our solution was made according to the following:


  • 22.2g of CaCl2

    • The buffer should be made fresh for each sample that is to be run, with the total volume equalling 20mL in a 50mL beaker. The way to make the buffer is as follows:


    1. Add 1mL of the NaCl
    2. Add 1mL of the NaH2PO4
    3. Add (18-X)mL of nanopure water, where X is the amount in mL of sample to be measured (This brings the pH to about 8.6)



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