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Team:Calgary/Notebook/Protocols/Process26
From 2011.igem.org
Notebook
Post-Regionals
Journal
- Defining Objectives
- Techniques Workshop
- Bioinformatics & qRT-PCR
- Sensory Element Fishing
- Electrochem - CPR Oxidation
- Electrochem - Reporter Gene
- Pseudomonas Tools
- Microalgae Tools
- NA Viability Assays
- May - July Group Journal
Protocols
Safety
Buffer Preparation
The electrode and the sample to be measured need to be in a solution that is conductive to electricity to be able to measure the oxidation. To make our buffer, we used calcium chloride dissolved in nanopure water the most effective concentration was found to be at 0.1M. Our solution was made according to the following:
The buffer should be made fresh for each sample that is to be run, with the total volume equalling 20mL in a 50mL beaker. The way to make the buffer is as follows:
- Add 1mL of the NaCl
- Add 1mL of the NaH2PO4
- Add (18-X)mL of nanopure water, where X is the amount in mL of sample to be measured (This brings the pH to about 8.6)
