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Post-Regionals

Journal

• Defining Objectives
• Techniques Workshop
• Bioinformatics & qRT-PCR
• Sensory Element Fishing
• Electrochem - CPR Oxidation
• Electrochem - Reporter Gene
• Pseudomonas Tools
• Microalgae Tools
• NA Viability Assays
• May - July Group Journal

Protocols

Safety

Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.

Microalgae Protocols

• Preparation of Algal f2 Media
• Glass Bead Algal transformation
• Chloroplast Isolation
• Nuclear DNA extraction from Algae

Pseudomonas Biotinylation Techniques

• Plasmid Extraction (from Pseudomonas spp.)
• Biotinylation/Immunoprecipitation of Naphthenic Acids
• Conjugation of E. coli plasmids to Pseudomonas spp..

General Protocols

• Plasmid Isolation (from E. coli)
• RNA Extraction
• cDNA Synthesis
• Quantitative Real Time PCR
• Testing the pBAD-LacZ Construct
• Taq PCR Protocol
• PCR Purification
• Gel Extraction
• Testing the LacI-LacZ I732901 gene
• Bacterial Transformation
• Rehydration of Registry DNA
• Construction Techniques
• Agarose Gel Electrophresis
• LB Agar Plates
• Overnight Cultures
• Preparing chemically competent cells (E. coli)
• Preparing glycerol stocks (E. coli)