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From 2011.igem.org

Week 1: 2nd - 8th may

Bisphenol A:

• cloning of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> behind weak (<partinfo>J23103</partinfo>) and medium strong (<partinfo>J23110</partinfo>) constitutive promoter (each part and both parts polycistronic)
• cloning of fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo>, also assembly behind weak and medium strong constitutive promoter
• testing and establishing of HPLC method for BPA detection
• expression of the successfully assembled BPA degrading biobricks in E. coli TOP10

S-layer:

• successful PCR on the S-layer genes of B. flavum and C. crenatum
• successful cloning of the S-layer gene of B. flavum without TAT-sequence

Organizational:

• meeting with Bielefeld Marketing to discuss our participation at the science festival [http://www.geniale-bielefeld.de/ GENIALE] in Bielefeld

Week 2: 9th - 15th may

Bisphenol A:

• cloning of <partinfo>K157011</partinfo> behind existing BPA degrading parts (for purification and testing of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> in a cell free system)
• HPLC results: fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> can degrade BPA

S-layer:

• expression of the S-layer genes in E. coli

Organizational:

• moving to our own room in the [http://www.cebitec.uni-bielefeld.de/ CebiTec]