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Team:Bielefeld-Germany/Labjournal
From 2011.igem.org
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Organizational: | Organizational: | ||
| - | * meeting with Bielefeld Marketing to discuss our participation at the science festival [http://www.geniale-bielefeld.de/ GENIALE] in Bielefeld | + | * meeting with [http://www.bielefeld-marketing.de/de/index.html Bielefeld Marketing] to discuss our participation at the science festival [http://www.geniale-bielefeld.de/ GENIALE] in Bielefeld |
=Week 2: 9th - 15th may= | =Week 2: 9th - 15th may= | ||
Bisphenol A: | Bisphenol A: | ||
* cloning of <partinfo>K157011</partinfo> behind existing BPA degrading parts (for purification and testing of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> in a cell free system) | * cloning of <partinfo>K157011</partinfo> behind existing BPA degrading parts (for purification and testing of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> in a cell free system) | ||
| - | * HPLC results: fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> can degrade BPA | + | * HPLC results: fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> can degrade BPA and seems to work better than the polycistronic version |
S-layer: | S-layer: | ||
| - | * expression of the S-layer | + | * expression of the S-layer gene of ''B. flavum'' in ''E. coli'' |
Organizational: | Organizational: | ||
* moving to our own room in the [http://www.cebitec.uni-bielefeld.de/ CeBiTec] | * moving to our own room in the [http://www.cebitec.uni-bielefeld.de/ CeBiTec] | ||
Revision as of 16:56, 12 May 2011
Week 1: 2nd - 8th may
Bisphenol A:
- cloning of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> behind weak (<partinfo>J23103</partinfo>) and medium strong (<partinfo>J23110</partinfo>) constitutive promoter (each part and both parts polycistronic)
- cloning of fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo>, also assembly behind weak and medium strong constitutive promoter
- testing and establishing of HPLC method for BPA detection
- expression of the successfully assembled BPA degrading biobricks in E. coli TOP10
S-layer:
- successful PCR on the S-layer genes of B. flavum and C. crenatum
- successful cloning of the S-layer gene of B. flavum without TAT-sequence
Organizational:
- meeting with [http://www.bielefeld-marketing.de/de/index.html Bielefeld Marketing] to discuss our participation at the science festival [http://www.geniale-bielefeld.de/ GENIALE] in Bielefeld
Week 2: 9th - 15th may
Bisphenol A:
- cloning of <partinfo>K157011</partinfo> behind existing BPA degrading parts (for purification and testing of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> in a cell free system)
- HPLC results: fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> can degrade BPA and seems to work better than the polycistronic version
S-layer:
- expression of the S-layer gene of B. flavum in E. coli
Organizational:
- moving to our own room in the [http://www.cebitec.uni-bielefeld.de/ CeBiTec]
