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Team:Bielefeld-Germany/Labjournal
From 2011.igem.org
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=Week 1: 2nd - 8th may= | =Week 1: 2nd - 8th may= | ||
Bisphenol A: | Bisphenol A: | ||
| - | * cloning of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> behind weak and medium strong constitutive promoter (each part and both parts polycistronic) | + | * cloning of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> behind weak (<partinfo>J23103</partinfo>) and medium strong (<partinfo>J23110</partinfo>) constitutive promoter (each part and both parts polycistronic) |
* cloning of fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo>, also assembly behind weak and medium strong constitutive promoter | * cloning of fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo>, also assembly behind weak and medium strong constitutive promoter | ||
* testing and establishing of HPLC method for BPA detection | * testing and establishing of HPLC method for BPA detection | ||
Revision as of 14:38, 9 May 2011
Week 1: 2nd - 8th may
Bisphenol A:
- cloning of <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> behind weak (<partinfo>J23103</partinfo>) and medium strong (<partinfo>J23110</partinfo>) constitutive promoter (each part and both parts polycistronic)
- cloning of fusionprotein between <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo>, also assembly behind weak and medium strong constitutive promoter
- testing and establishing of HPLC method for BPA detection
- expression of the successfully assembled BPA degrading biobricks in E. coli TOP10
S-layer:
- successful PCR on the S-layer genes of B. flavum and C. crenatum
- successful cloning of the S-layer gene of B. flavum without TAT-sequence
Week 2: 9th - 15th may
Bisphenol A:
- cloning of <partinfo>K157011</partinfo> behind existing BPA degrading parts
S-layer:
- expression of the S-layer genes in E. coli
