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  • Team:Debrecen Hungary/Protocols
    6) Close it and stick a tape from one side of the wall vertically over the ca 6. Spread the transformed bacteria on the Petri dishes with LB agar and the a
    51 KB (7,937 words) - 05:49, 22 September 2011
  • Team:WHU-China/calendarjs
    ...nction Fa(a,b,e){for(b=b||1;!a.getDay()||e&&a.getDay()==1||!e&&a.getDay()==6;)ba(a,b);return a}function Ca(a,b){return Math.round((N(a,true)-N(b,true))/ ...)%7);Q=Math.round((S-y)/(Ab*7));if(f("weekMode")=="fixed"){ba(S,(6-Q)*7);Q=6}d.title=l(j,
    72 KB (13,661 words) - 13:53, 24 October 2011
  • Team:Penn/project/protocols/
    ...='http://benjaminshyong.com/igem2011/wp-includes/js/jquery/jquery.js?ver=1.6.1'></script> 6. Add 1.5 mL trypsin to the bottom of the flask. Move the flask back and for
    86 KB (13,981 words) - 04:46, 29 September 2011
  • Team:OUC-China/Result/week12
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> ...re used for standerdizing 18-3-11,14-19,5-4,rhiABCpro,13-6-leu and 18-3-11-6-leuB have already bacteria. And we select some into LB.</p>
    7 KB (1,143 words) - 02:38, 29 October 2011
  • Team:Potsdam Bioware/Labjournal/September part 2
    * 6 overnight cultures | mdnABCDE with T7-promotor clone 1 || 50.6
    58 KB (8,180 words) - 00:26, 22 September 2011
  • Team:TU Munich/lab/notebook/part3
    ...dinburgh part") conc: 150 ng/µl</p><p>primers (synthesized by biomers, 29.6.11, stock: 100µM):</p> ...iPrep iGEM R2 31.5.11) was digested with X,P and T7prom (black writing: 17.6.11 T7) with S,P. After heat-inactivation, GLP was added to the digested DNA
    121 KB (20,136 words) - 01:54, 22 September 2011
  • Team:WashU/Notebook/June2011
    == June 6 == **[[File:IGEM Practice Gel 6-9.jpg|thumb|none|upright=3]]
    26 KB (4,070 words) - 16:08, 25 July 2011
  • Team:Harvard/Template:NotebookData4
    *these were the only two PCR reactions from 6/22/11 to work **omega: 6.1ng/µL, 260/280=1.83
    31 KB (4,866 words) - 16:56, 8 August 2011
  • Team:WHU-China/Notebook/Daytoday
    <span style="font-weight:bolder">5/29~6/1</span>, use Pst1 and EcoRⅠto digest pDsRed-Express Vector</br> <span style="font-weight:bolder">6/2~6/15</span>, produce the competent cell, use T4 ligase to join DNA fragments,
    29 KB (3,834 words) - 14:34, 26 October 2011
  • Team:Rutgers/LB1
    <td colspan="6" td background="https://static.igem.org/mediawiki/2011/9/96/Stripe.png"><h1 <td colspan="6" class="stuff">
    62 KB (8,401 words) - 02:39, 29 September 2011
  • Team:TU Munich/lab/notebook/part4
    <p>6: pSB4A5, c = 175 ng/µl</p> <td>6: BBa_K568001 </td><td><span style="color:#FFAA00">6.8 kbp</span> </td><td>2 kbp </td><td>E,P
    78 KB (13,436 words) - 22:42, 28 October 2011
  • Team:Harvard/Template:NotebookData2
    **Bands followed the same pattern as the gel run on 6/13/11 **OZ052 (from 1:100 dilution): 20.6 ng/µL, 260/280=2.17
    26 KB (3,991 words) - 23:44, 25 October 2011
  • Team:NCTU Formosa/calendar
    ...ckbone to 1C3 5. extract plasmid of D circuit and check by electrophoresis 6. incubated (B0034+ilvC) (B0030+ilvD) (B circuit 7-1) (K098995 +B0034) (vioA
    46 KB (7,856 words) - 13:40, 5 October 2011
  • Team:BU Wellesley Software/Notebook/SiruiNotebook
    *'''5/31-6/1''': Team introductions; we learned the basics of CAD and basics of molecu *'''6/2''': We started learning the basics of Clotho, as well as more basic biolo
    29 KB (4,439 words) - 03:57, 29 September 2011
  • Team:BU Wellesley Software/Notebook/ChenkaiNotebook
    *'''Wednesday 6/8''' <h2>Thursday, 6/9</h2>[[File: RMPuppeteerCommands 6-9-11.png |300px|thumb|right|New Puppeteer output with UUIDS replacing sampl
    28 KB (4,611 words) - 16:34, 27 September 2011
  • Team:Harvard/Template:NotebookData
    *6) 68⁰C for 5 min ...hould have been 1200 µL bacteria media, 300 µL 80% glycerol ''(Corrected 6/14/2011)'' ''' (80% pure glycerol, 20% molecular grade water)
    14 KB (1,886 words) - 14:52, 12 August 2011
  • Team:Grinnell/Notebook/Gels/Esp
    ...> ligated with <i>rsaA</i> C-terminal PCR product from 20110605. Only lane 6 shows successful ligation of <i>esp</i> and <i>rsaA</i> C-terminal. ...sted 20110606; lane 5: PCR product of plasmid containing <i>esp</i>; lanes 6-8: PCR product using VF2 and VR of plasmid containing <i>esp</i> ligated wi
    32 KB (4,737 words) - 21:32, 1 August 2011
  • Team:Harvard/Template:NotebookDataJuly
    ... We grew up 12 colonies from each 6/29 plate and 6 "colonies" from the 2mL 6/30 plate in SOC medium with kanamycin, and next week we will PCR the 152962 **Used KAPA master mix and protocols, same procedure as 6/29/11
    9 KB (1,439 words) - 18:13, 3 August 2011
  • Team:Potsdam Bioware/Labjournal/June
    | Primer (each) || 3 || 0.6 µM | 6 || || Hold
    70 KB (9,678 words) - 01:31, 22 September 2011
  • Team:Grinnell CN/Notebook/Gels/Esp
    ...> ligated with <i>rsaA</i> C-terminal PCR product from 20110605. Only lane 6 shows successful ligation of <i>esp</i> and <i>rsaA</i> C-terminal. ...sted 20110606; lane 5: PCR product of plasmid containing <i>esp</i>; lanes 6-8: PCR product using VF2 and VR of plasmid containing <i>esp</i> ligated wi
    32 KB (4,738 words) - 04:14, 27 September 2011

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