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Mutagenesis PCR
From 2011.igem.org
Mutagenesis PCR was done using the protocol established by agilent's Quiklightning Multi-site directed mutagenesis kit.
Our Mutagenesis Primers were:
1. 5' - CGAATCACGTGCTACAGCGCGCCTACG - 3'
2. 5' - ACCTGCCGCACTGTAGTTTCTCGCGGG - 3'
3. 5' - CTCGACCGAACACCTACAGGGCGACTTCTAC - 3'
