Copenhagen/2 August 2011

From 2011.igem.org

(Difference between revisions)
(Tuesday)
 
(4 intermediate revisions not shown)
Line 6: Line 6:
* Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.  
* Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.  
 +
 +
*Image of gel:
 +
 +
[[image:PCR gel 2-8-11.jpg|400px|right]]
 +
* Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.
* Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.
* Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.
* Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.
 +
 +
* PCR on the A1 and B1 from the plates from yesterday - to see if its our procedure thats wrong or there's simply no insert. At the same time we are checking our "old" B1 from the 25/26th of July in the expr. vector: 3 from transformed XLblue cells  and 3 colonies from transformed Bl21 cells. 

Latest revision as of 07:36, 3 August 2011

Tuesday

We are very excited about the gel we are going to make today!!

Labwork

  • Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.
  • Image of gel:
PCR gel 2-8-11.jpg


  • Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.
  • Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.
  • PCR on the A1 and B1 from the plates from yesterday - to see if its our procedure thats wrong or there's simply no insert. At the same time we are checking our "old" B1 from the 25/26th of July in the expr. vector: 3 from transformed XLblue cells and 3 colonies from transformed Bl21 cells.


Other work

  • Renew projectdescription
  • Find a fungi induced promoter


Back to Notebook