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From 2011.igem.org

Tuesday

We are very excited about the gel we are going to make today!!

Labwork

• Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.

• Image of gel:

• Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.

• Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.

• PCR on the A1 and B1 from the plates from yesterday - to see if its our procedure thats wrong or there's simply no insert. At the same time we are checking our "old" B1 from the 25/26th of July in the expr. vector: 3 from transformed XLblue cells and 3 colonies from transformed Bl21 cells.

Other work

• Renew projectdescription

• Find a fungi induced promoter

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