Copenhagen/29 July 2011

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Revision as of 09:44, 29 July 2011

Friday

The controls we made with the expression vector seem to work. We only had six colonies on the two controls C1 and C2 but many more on the plates with inserts.

It is worse with the psb1c3. Here we have almost eqaul amount of colonies on the controls plates and on the ones with insert. If the insert is ligated into the plasmid the colony should be white compared with them without, which are pink. It is difficult to determine the colour on them with insert.

Lab work

colony-PCR on the colonies from the ligation

run a gel to check if the ligations are succeded

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 '''Friday'''
-The controls we made with the expression vector seems to work. We only had six colonies on the two controls C1 and C2 and many more on the plates with inserts.
+The controls we made with the expression vector seem to work. We only had six colonies on the two controls C1 and C2 but many more on the plates with inserts.
-It is worse with the psb1c3. Here we have almost eqaul amount of colonies on the controls plates and on the ones with insert. If the insert is ligated into the plasmid the colony should be white compared with them without, with are pink. It is difficult to determine the colour on them with insert.
+It is worse with the psb1c3. Here we have almost eqaul amount of colonies on the controls plates and on the ones with insert. If the insert is ligated into the plasmid the colony should be white compared with them without, which are pink. It is difficult to determine the colour on them with insert.
 '''Lab work'''