Copenhagen/28 June 2011

From 2011.igem.org

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* Finished the mutation: Digested the parental plasmid. Protocol [[Team:Copenhagen/Protocol#Mutations|Mutations]]
* Finished the mutation: Digested the parental plasmid. Protocol [[Team:Copenhagen/Protocol#Mutations|Mutations]]
* Transformed E.coli with our new mutant plasmids. Protocol [[Team:Copenhagen/Protocol#Transformations|Transformations]]
* Transformed E.coli with our new mutant plasmids. Protocol [[Team:Copenhagen/Protocol#Transformations|Transformations]]
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* Took one colony out of our ''XL1-Blue'' transformed with biobrick no. BBa_J04500 [http://partsregistry.org/Part:BBa_J04500] and placed it in an overnight culture.
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* Took one colony out of our ''XL1-Blue'' transformed with biobrick no. BBa_J04500 [http://partsregistry.org/Part:BBa_J04500|BBa_J04500] and placed it in an overnight culture.
===Tomorrow===
===Tomorrow===
We will do Real Fast Mini Preps of our BioBrick and hopefully be able to pick a colony transformed with our cyp for an overnight culture.
We will do Real Fast Mini Preps of our BioBrick and hopefully be able to pick a colony transformed with our cyp for an overnight culture.

Revision as of 10:03, 28 June 2011

Contents

Thuesday

Status

It seems that something went worng with our mutants from last week since we weren't able to transform XL1-Blue with them - even though we used a larger amount of plasmid. So now we put all our faith in the mutants we made yesterday.

Lab work

  • Finished the mutation: Digested the parental plasmid. Protocol Mutations
  • Transformed E.coli with our new mutant plasmids. Protocol Transformations
  • Took one colony out of our XL1-Blue transformed with biobrick no. BBa_J04500 [http://partsregistry.org/Part:BBa_J04500|BBa_J04500] and placed it in an overnight culture.

Tomorrow

We will do Real Fast Mini Preps of our BioBrick and hopefully be able to pick a colony transformed with our cyp for an overnight culture.