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From 2011.igem.org

Contents 1 Thuesday 1.1 Status 1.2 Lab work 1.3 Tomorrow

Thuesday

Status

It seems that something went worng with our mutants from last week since we weren't able to transform XL1-Blue with them - even though we used a larger amount of plasmid. So now we put all our faith in the mutants we made yesterday.

Lab work

• Finished the mutation: Digested the parental plasmid. Protocol Mutations
• Transformed E.coli with our new mutant plasmids. Protocol Transformations
• Took one colony out of our XL1-Blue transformed with biobrick no. BBa_J04500 [1] and placed it in an overnight culture.

Tomorrow

We will do Real Fast Mini Preps of our BioBrick and hopefully be able to pick a colony transformed with our cyp for an overnight culture.

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