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Copenhagen/28 June 2011
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===Status=== | ===Status=== | ||
| - | It seems that something went worng with our mutants from last week since we weren't able to transform '' | + | It seems that something went worng with our mutants from last week since we weren't able to transform ''XL1-Blue'' with them - even though we used a larger amount of plasmid. |
So now we put all our faith in the mutants we made yesterday. | So now we put all our faith in the mutants we made yesterday. | ||
===Lab work=== | ===Lab work=== | ||
| - | * Finished the mutation: Digested the parental plasmid | + | * Finished the mutation: Digested the parental plasmid. Protocol [[Team:Copenhagen/Protocol#Mutations|Mutations]] |
| - | * Transformed E.coli with our new mutant plasmids. Protocol | + | * Transformed E.coli with our new mutant plasmids. Protocol [[Team:Copenhagen/Protocol#Transformations|Transformations]] |
| - | * Took one colony out of our XL1-Blue transformed with biobrick no. BBa_J04500 and placed it in an overnight culture. | + | * Took one colony out of our ''XL1-Blue'' transformed with biobrick no. BBa_J04500 [http://partsregistry.org/Part:BBa_J04500] and placed it in an overnight culture. |
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| + | ===Tomorrow=== | ||
| + | |||
| + | We will do Real Fast Mini Preps of our BioBrick and hopefully be able to pick a colony transformed with our cyp for an overnight culture. | ||
| + | |||
| + | |||
| + | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Latest revision as of 10:05, 28 June 2011
Contents |
Thuesday
Status
It seems that something went worng with our mutants from last week since we weren't able to transform XL1-Blue with them - even though we used a larger amount of plasmid. So now we put all our faith in the mutants we made yesterday.
Lab work
- Finished the mutation: Digested the parental plasmid. Protocol Mutations
- Transformed E.coli with our new mutant plasmids. Protocol Transformations
- Took one colony out of our XL1-Blue transformed with biobrick no. BBa_J04500 [1] and placed it in an overnight culture.
Tomorrow
We will do Real Fast Mini Preps of our BioBrick and hopefully be able to pick a colony transformed with our cyp for an overnight culture.
Back to Notebook
