Copenhagen/15 July 2011

From 2011.igem.org

(Difference between revisions)
(Lab Work)
 
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===Lab Work===
===Lab Work===
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'''A1'''
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* We purified A1 with [[Team:Copenhagen/Protocol#Mini_prep|Mini prep]] from 3 different colonies.
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* We analyzed A1 [[Team:Copenhagen/Protocol#Restrictionsite_analysis|cut]] with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. The results looked good and we sent them to be sequenced.
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'''A2'''
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*[[Team:Copenhagen/Protocol#Double Digest|Double digestion]] of A1 using Kenneths approach.
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* The plasmid was purified with [[Team:Copenhagen/Protocol#Gel_Extraction_Protocol|gel purification]]
'''B1'''
'''B1'''
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===Other work===
===Other work===
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Team building and listening to reggae.
Back to [[Team:Copenhagen/Notebook|Notebook]]
Back to [[Team:Copenhagen/Notebook|Notebook]]

Latest revision as of 14:14, 18 July 2011

Friday

Deadline for preliminary project description and safety page

Lab Work

A1

  • We purified A1 with Mini prep from 3 different colonies.
  • We analyzed A1 cut with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. The results looked good and we sent them to be sequenced.

A2

B1

We run a PCR to confirm that colony contains CYP79B1.

We plate the colony once again on agarplates with chloramphenicol hoping to collect a lot of cells after the weekend:)

Other work

Team building and listening to reggae.

Back to Notebook