Copenhagen/15 July 2011
From 2011.igem.org
Friday
Deadline for preliminary project description and safety page
Lab Work
A1
- We purified A1 with Mini prep from 3 different colonies.
- We analyzed A1 cut with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. The results looked good and we sent them to be sequenced.
A2
- Double digestion of A1 using Kenneths approach.
- The plasmid was purified with gel purification
B1
We run a PCR to confirm that colony contains CYP79B1.
We plate the colony once again on agarplates with chloramphenicol hoping to collect a lot of cells after the weekend:)
Other work
Team building and listening to reggae.
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