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Copenhagen/15 July 2011
From 2011.igem.org
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===Lab Work=== | ===Lab Work=== | ||
| + | |||
| + | '''A1''' | ||
| + | |||
| + | * We purified A1 with [[Team:Copenhagen/Protocol#Mini_prep|Mini prep]] from 3 different colonies. | ||
| + | * We analyzed A1 [[Team:Copenhagen/Protocol#Restrictionsite_analysis|cut]] with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. The results looked good and we sent them to be sequenced. | ||
| + | |||
| + | '''A2''' | ||
| + | |||
| + | *[[Team:Copenhagen/Protocol#Double Digest|Double digestion]] of A1 using Kenneths approach. | ||
| + | * The plasmid was purified with [[Team:Copenhagen/Protocol#Gel_Extraction_Protocol|gel purification]] | ||
'''B1''' | '''B1''' | ||
| - | We run a PCR to confirm that colony contains CYP79B1. | + | We run a [[Team:Copenhagen/Protocol#PCR of BioBrick in pSB1C3|PCR]] to confirm that colony contains CYP79B1. |
We plate the colony once again on agarplates with chloramphenicol hoping to collect a lot of cells after the weekend:) | We plate the colony once again on agarplates with chloramphenicol hoping to collect a lot of cells after the weekend:) | ||
| - | |||
===Other work=== | ===Other work=== | ||
| + | Team building and listening to reggae. | ||
Back to [[Team:Copenhagen/Notebook|Notebook]] | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Latest revision as of 14:14, 18 July 2011
Friday
Deadline for preliminary project description and safety page
Lab Work
A1
- We purified A1 with Mini prep from 3 different colonies.
- We analyzed A1 cut with Pst1 (which cut at the restrictionsite we want to remove)on an agarose gel. The results looked good and we sent them to be sequenced.
A2
- Double digestion of A1 using Kenneths approach.
- The plasmid was purified with gel purification
B1
We run a PCR to confirm that colony contains CYP79B1.
We plate the colony once again on agarplates with chloramphenicol hoping to collect a lot of cells after the weekend:)
Other work
Team building and listening to reggae.
Back to Notebook
