Team:Paris Liliane Bettencourt/Notebook/2011/07/05/

From 2011.igem.org

Team IGEM Paris 2011

ligation and transformation

Axel

ligation

  • BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
  • BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL

Babak

Gel purification

  • well 1: ladder 100pb
  • from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL

Digestion

  • BBa_K090403 cut by EcoR1 and XbaI
  • BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx
  • BBa_J18913,2428pb, cut by EcoR1 and XbaI
  • BBa_pHyperspank,121pb, cut by Xbal and Ptsx
  • B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI

Mathias & Laura

After sequencing, we could see the BioBrick the parts registry sent us under the name K143033 was not LacI but a sorbitol so we have to redo the construction the parts LacI+ double terminator.

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