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Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy
From 2011.igem.org
Microscopy test
This protocol aims at preparing glass slides for observation of biofilms with a fluorescent microscope.
Procedure
1. In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.
2. Add 100µL of bacteria from a saturated liquid culture.
3. Finally, add 3 or 4 sterile glass slides with a sterile tweezer.
4. Incubate at 30°C overnight.
5. Observe the slides with a fluorescent microscope.
