Team:Freiburg/Notebook/18 July
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Precipitator
PCR
Name:
Ruediger | Date:
18.07.2011 |
Continue from Experiment (Date)PCR 1507
(Name) Ruediger | |
Project Name:
GFP Pbd |
PCR-Mixture for one Reaction:
For a 50 µl reaction use
32,5µl | H20 | |
10µl | 5x Phusion Buffer | |
2.5µl | Primer fw | |
2.5µl | Primer dw | |
1µl | dNTPs | |
1µl | DNA-Template | |
0.5 µl | Phusion (add in the end) |
What program do you use?
10x (95C-41C/47C/52C-72C) + 25x ((95C-60C-72C)
How did you label the PCR-Product, where is it stored and what do you do next?
Reactions:
P28+P18+M14.1
P28+P19+M14.1
P28+P20+M14.1
P28+P2+M14.1
P28+P3+M14.1
P1+P18+M14.1
P1+P19+M14.1
P1+P20+M14.1
P1+P2+M14.1
P1+P3+M14.1
P1+P3+M14.2
Upper panel:All lanes at 41C
Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | Lane 10 |
Quick Load Marker | P28+P18+M14.1 | P28+P19+M14.1 | P28+P20+M14.1 | P28+P2+M14.1 | P1+P19+M14.1 | P1+P20+M14.1 | P1+P2+M14.1
|
Lower Panel
Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | Lane 10 |
Quick Load Marker | P1+P3+M14.1
41C | P1+P3+M14.1
47C | P1+P3+M14.2
52C | P1+P3+M14.1
52C | P28+P3+M14.1
41C | P1+P18+M14.1
41C | P28+P3+M14.1
47C | P28+P3+M14.1
52C |
Upper Panel: all at 47C
Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | Lane 10 |
Quick Load Marker | P28+P18+M14.1 | P28+P19+M14.1 | P28+P20+M14.1 | P28+P2+M14.1 | P1+P18+M14.1 | P1+P19+M14.1 | P1+P20+M14.1 | P1+P2+M14.1 |
Lower Panel: all at 52C
Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | Lane 10 |
Quick Load Marker | P28+P18+M14.1 | P28+P19+M14.1 | P28+P20+M14.1 | P28+P2+M14.1 | P1+P18+M14.1 | P1+P19+M14.1 | P1+P20+M14.1 | P1+P2+M14.1 |
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